Bao Ge Liao Agar Girl: The Misadventures yes thats what i am. i just finished cutting up the whole roll of membranes (synthetic. not human) and it was a serious mistake on their part because i reputably cut, fold, draw lines crooked. really. very very crooked. i feel like a jack of all trades but master of none. i'm the glorified errands girl who runs a few procedures on her own. ok so maybe jack of all trades and master of none except doing pcr and running them on agar gel (carcinogenic and EtBr flavoured)...i do that almost every day. i don't chong kopi or deliver kai fan for my colleagues but i cut membranes 8.5cm x 6.5cm wash membrane 4 x 10 mins wash bottles (in smelly machine!) fill pipette tips in boxes move boxes from the oven to the cupboard but of course, when in luck, i get to do alot of fun stuff like *cast a few kinds of gels. (haha told you i was the agar girl) - sds page gel, gel electrophoresis, ief gel (which is pretty smelly belly) *coomasie for protein quantity analysis *extract (which includes syringing cells up n down a tube at least 50 times. for each tube) and analyse rna quantity. speaking of which, while doing that i broke a capillary in the quantifying machine and someone had to open up the machine just to take it out. oops? *sds page *western blotting *pcr *ligation *transfection, transformation *make cell culture that's all i remember. i think i havent mentioned all the mishaps apart from the capillary stuck in the machine incident lets see. i've stopped a colleague's pcr machine prematurely put in 10 ml instead of 1ml into the cells kept the rna that took 1 day to extract in the fridge, not freezer left a bottle of (someone else's) agar solution in rm temp so the whole bottle solidified used unsterilised pipette tips in the sterilised rna biosafety cupboard erm left footprints all over the lab? i dunnoe whats wrong with my shoe! i tried cleaning up while they weren't looking. i just had to let you know what ive been doing around here. for now, im really bored. because science makes you wait. machines make you wait. so i wait